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10-Minute Presentation

Systematics, Evolution, and Biodiversity

10-min: SysEB, Systematics, Phylogenetics, and Evolution 2

Phylogenetics using historical DNA from dried specimens: Possibilities and limitations in large (Cerambycidae) and small (Scolytinae) beetles

Tuesday, November 12, 2024
4:48 PM – 5:00 PM MST
Location: Phoenix Convention Center, 132 A, PCC
Oral Presentation

    Presenting Author(s)

  • Patrick S. Gorring, Ph.D.

    Curator of Coleoptera, Odonata, and Orthoptera
    Michigan State University
    East Lansing, Michigan

    • Co-Author(s)

  • AC

    Anthony I. Cognato

    Professor, Collection Director, and Graduate Advisor
    Michigan State University
    East Lansing, Michigan

Museums house a wealth of historical genetic information that, with modern techniques, can be extracted and used to explore phylogeny and evolutionary ecology. We extracted DNA from dried beetles as small as 1mm and have used enrichment and NGS sequencing to build useful DNA data matrices for phylogenetic estimation. We outline some of the ways we optimized the workflow for the special case of dried beetles up to a century old with differing levels of preservation. Using dried specimens should be possible for all insect groups and opens up the possibility of non-destructively sequencing rare, unobtainable, or extinct specimens.