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Section Symposium

Medical, Urban, and Veterinary Entomology

Physiology, Biochemistry, and Toxicology

At the Nexus of Ticks and Tick-Borne Illness Mitigation: Novel Perspectives for Tick Disease Control

Two types of axonal muscarinic acetylcholine receptors mediate formation of saliva cocktail in the tick Ixodes ricinus

Tuesday, November 12, 2024

10:20 AM – 10:35 AM MST

Location: Phoenix Convention Center, 221 C

Presenting Author(s)

LS

Ladislav Simo

Institut National de la Recherche Agronomique (INRAe)
Maisons-Alfort, Ile-de-France, France

Co-author(s)

CN

Caina Ning

Institut National de la Recherche Agronomique (INRAe)
Maisons-Alfort, Ile-de-France, France
JV

James Valdes

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
LM

Lourdes Mateos-Hernandez

Institut National de la Recherche Agronomique (INRAe)
Maisons-Alfort, Ile-de-France, France
SR

Sabine Rakotobe

Institut National de la Recherche Agronomique (INRAe)
Maisons-Alfort, Ile-de-France, France
LS

Livia Sofrankova

Institut National de la Recherche Agronomique (INRAe)
Maisons-Alfort, Ile-de-France, France
HF

Helena Frantova

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
VU

Veronika Urbanova

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
TK

Tereza Kozelkova

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
FD

Filip Dycka

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
PK

Petr Kopacek

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
OH

Ondrej Hajdusek

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
RS

Radek Sima

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
JT

Jiri Tyc

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
TB

Tomas Bily

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
MV

Marie Vancova

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
JP

Jan Perner

Czech Academy of Sciences
Ceske Budejovice, Jihocesky kraj, Czech Republic
ST

Steeve Thany

Institut National de la Recherche Agronomique (INRAe)
Orleans Cedex, Lorraine, France

The secretion of salivary gland (SG) in ticks is effectively triggered by the muscarinic acetylcholine receptor (mAChR) agonist, the pilocarpine, known as a worldwide-preferred tool for obtaining copious saliva. Presently, all we know is, that injection or apical application of pilocarpine, into/on partially-fed tick females induces robust long-lasting salivary secretion, whereas it fails to induce salivation from isolated SG. Cutting neuronal connections between tick synganglion and SG, support the involvement of a neural mAChR in these processes. Based on these observations, it has been generally believed that tick synganglion neurons, mediates SG fluid secretion via unidentified non-cholinergic “secreto-motor nerves”, connecting neuronal cells with SG. Currently, the only known processes, associating specific tick neurons with SG are the neuropeptidergic axons innervating either type II or both type II/III saliva-producing acini. Here, based on agonistic/antagonistic assessments along mutant-based verifications, we revealed that Ixodes ricinus tick, possesses two pharmacologically distinct types (-A and -B) of mAChR. The synganglion was suggested to sense cholinomimetics via rich axonal arborizations at its surface, originating from two clusters of peptidergic neurosecretory cells, each expressing either mAChR-A or -B. Furthermore, both sets of neuropeptidergic SG-innervating cells also possess a cholinoceptive feature by expressing either of these two receptor types on their terminals in saliva producing type II and III acini. Following this model, the effects of in vivo-injected selective muscarinic agents, put forward that co-joint action of two different axonal mAChRs mediate a tandem cooperation of type II/III SG acini for saliva cocktail formation.