D3152: Holobiont genomics for exploring the roles of small RNA in mosquito-microbe communication

In the quest to understand mosquito-microbe dynamics, we are exploring the role of the holobiont, and bacteria’s effects in mosquito germlines. Small RNAs are known regulators of gene expression and are hypothesized to facilitate communication between mosquitoes and their microbiome. Utilizing sequencing techniques for for 3’ end methylation, indicative of eukaryotic small RNAs, our lab identified a subset with sequence homology to known microbiome members in Anopheles stephensi. This finding suggests a potential pathway for inter-domain communication within the mosquito holobiont. The hypothesis suggests that small RNAs may originate from microbial commensals inhabiting the mosquito gut or tissues, subsequently processed by the mosquito's cellular machinery. Understanding this mechanism could shed light on how microbes influence mosquito physiology, immunity, and vector competence. Furthermore, it could unveil targets for disrupting pathogen transmission cycles. Deciphering small RNA-mediated communication underscores the importance of holobiont concepts in vector research, recognizing mosquitoes not merely as individual organisms but as complex ecosystems shaped by symbiotic relationships. This holistic perspective may lead to innovative interventions to mitigate mosquito-borne diseases' global health burden. In essence, investigating small RNAs in mosquito-microbe interactions offers promise for advancing our understanding of vector biology and developing effective disease control strategies