D3039: Conserved splicing of sex-determination isoforms in Diptera

Canonical sex-differentiation pathways in Diptera involve a sex-specific splicing cascade of mRNA, which results in sexually dimorphic characteristics. True flies of Diptera have independently evolved blood-feeding behavior on multiple occassions, and often show sexually-dimorphic blood-feeding behaviors. Control methods, such as genetic approaches (gene drive) and sterile insect technique, have been demonstrated as effective means to control wild and caged populations of arthropods. Understanding the
conservation of sex-specifc splicing occurs in dipteran pests is crucial, as it provides potential targets for genetic control of vector and pest arthropod species. We present
transcriptomic analysis showing evolutionary divergence of dsx, transformer (tra1/
tra2), and fruitless (fru) splicing in select dipterans, along with evidence of novel
sex-specifc splicing events. These findings could guide future genetic pest control
strategies and enhance our understanding of sex-specifc splicing in arthropod
speciation.