Poster Display
Physiology, Biochemistry, and Toxicology
Lixiang Wang
Zhengzhou University
Zhengzhou, Henan, China (People's Republic)
Zhongyuan Deng
Zhengzhou University
Zhengzhou, Henan, China (People's Republic)
Qian Ding
Zhengzhou University
Zhengzhou, Henan, China (People's Republic)
Xianchun Li, Ph.D. (he/him/his)
Professor
University of Arizona
Tucson, Arizona
The ATP-binding cassette (ABC) transporter family is one of the largest transporter families, which belong to a class of detoxifying enzymes in the third stage of insect metabolism and may play a key role in insecticide resistance. Here, we showed that verapamil, an inhibitor of ABC transporters, could significantly enhance the toxicity of emamectin benzoate (EB) to the model insect Drosophila melanogaster. Through the GAL4/UAS binary expression system, forty-six ABC transporter genes were knocked down in D. melanogaster. The subsequent bioassay result showed that silencing four ABC transporter genes could increase the mortality of D. melanogaster to EB. Based on the transcriptome and genome of Spodoptera frugiperda, five homologous genes (SfABCC4, SfABCG1, SfABCG4, SfABCG20 and SfABCG23) were identified. Spatiotemporal expression pattern analysis showed that SfABCC4 and SfABCG1 were highly expressed in the hemolymph. SfABCG4, SfABCG20 and SfABCG23 were predominately expressed in the head, midgut and integument. EB exposure induced upregulation of SfABCC4, SfABCG1, SfABCG4, SfABCG20 and SfABCG23 expression at different levels in S. frugiperda. Furthermore, overexpression of these five ABC transporters increased the viability of Sf9 cells under EB treatment. Our findings indicate that SfABCC4, SfABCG1, SfABCG4, SfABCG20 and SfABCG23 might involve in the detoxification of EB in S. frugiperda. These results are helpful in understanding the role of ABC transporters in EB resistance of other agricultural pests.