D3343: Insecticide resistance in Aedes aegypti populations of Ponce, Puerto Rico

Tuesday, November 12, 2024

9:00 AM – 5:00 PM MST

Location: Phoenix Convention Center, Exhibit Hall 6, PCC

Presenting Author(s)

Kristen Joy Adkins

PHEFA Fellow
Centers for Disease Control and Prevention / Entomological Society of America
Dover, Delaware

Co-Author(s)

GF

Gilberto Felix

Centers for Disease Control and Prevention
San Juan, Puerto Rico
MD

Marta Iris Diaz-Garcia

Centers for Disease Control and Prevention
San Juan, Puerto Rico
LR

Luis Rivera

Centers for Disease Control and Prevention
San Juan, Puerto Rico
Roberto Barrera, Ph.D.

Research Entomologist
Centers for Disease Control and Prevention
San Juan, Puerto Rico
Reinaldo L. Rivera Figueroa (he/him/his)

PHEFA Fellow
Centers for Disease Control and Prevention
Ponce, Puerto Rico
JR

Jose Ruiz-Valcarcel

Centers for Disease Control and Prevention
San Juan, Puerto Rico
RH

Ryan R. Hemme

Research Biologist
Centers for Disease Control and Prevention
Carolina, Puerto Rico

Aedes aegypti, the vector of dengue, chikungunya, and Zika viruses, has developed widespread resistance to insecticides across Puerto Rico. The recurrent use of the same class of insecticides can lead to resistance in successive generations. Here we present preliminary findings on insecticide resistance in mosquito populations collected in southern Puerto Rico. Nine sites were selected from “The Communities Organized to Prevent Arboviruses (COPA)” cohort in Ponce, PR. Between 80 and 120 ovitraps were deployed in each site using 10% and 100% hay infusion concentrations and collected after three days. Five insecticides (deltamethrin, alpha-cypermethrin, lambda-cyhalothrin, permethrin, and pyrethrum) were chosen for CDC bioassay testing using the CONUS manual. Female F¹ mosquitoes from all sampling sites were exposed to each insecticide at the determined diagnostic dose for 120 minutes. Mortality was recorded at diagnostic time and 120 minutes. Resistance to alpha-cypermethrin, pyrethrum, and permethrin at diagnostic times were high with less than 10% mortality across all sampled populations. Deltamethrin was more effective; however, the highest recorded mortality for this chemical was 27% at diagnostic time. Lambda-cyhalothrin showed the least resistance at diagnostic time with mortality ranging from 18% up to 89% among the sampled populations. The findings highlight the need for additional monitoring of insecticide resistance and the need for new chemicals with different modes of action as those to the pyrethroids.