D3331: Preliminary profile of gut microbiota in 3rd instar larvae of stable fly and substrate in Nebraska by shotgun sequencing

Tuesday, November 12, 2024

9:00 AM – 5:00 PM MST

Location: Phoenix Convention Center, Exhibit Hall 6, PCC

Presenting Author(s)

JW

John Wang

Research Entomologist
USDA-ARS
Lincoln, Nebraska

Co-Author(s)

Jerry Jerry Zhu

Research Entomologist
USDA-ARS
Lincoln, Nebraska
DB

David Boxler

Professor
University of Nebraska
Lincoln, Nebraska
Gary Brewer (he/him/his)

Professor
University of Nebraska
Lincoln, Nebraska

Stable flies (Stomoxys calcitrans L.) are a global livestock pest. Recent outbreaks have been attributed to the expending in available plant-based substrates for larval development. The 16S sequencing has less power as compared to shotgun sequencing when identifying less abundant taxa, although 16S is commonly used to characterize the microbial communities in many studies related to the important insect pests, including stable flies. Here, with shotgun sequencing, we investigated the microbial diversity in both the 3^rd instar larvae gut and supporting substrate within and from different locations.