The oriental fruit fly (Bactrocera dorsalis) is an invasive pest that infests a broad range of agricultural fruits and vegetables. Insect neuropeptides (NPs) and their specific G-protein coupled receptors (GPCRs) have been considered as biological targets for pest control because they play critical roles in nearly all vital physiological processes throughout insect life stages. In this talk, we provide updates on the identification and characterization of the PRXamide peptides and their corresponding receptors in B. dorsalis. The gene expressions were monitored across all life stages and in various tissues and organs. A comparative functional analysis of the GPCRs expressed in insect Sf9 cells was conducted to determine binding affinities with various peptides including the natural ligand in the in vitro system. Our results enhance the understanding of the potential biological functions of the target NP in B. dorsalis and may contribute to developing novel pest control methods for this species in the future.